Root spatial metabolite profiling of two genotypes of barley (Hordeum vulgare L.) reveals differences in response to short-term salt stress
نویسندگان
چکیده
Barley (Hordeum vulgare L.) is the most salt-tolerant cereal crop and has excellent genetic and genomic resources. It is therefore a good model to study salt-tolerance mechanisms in cereals. We aimed to determine metabolic differences between a cultivated barley, Clipper (tolerant), and a North African landrace, Sahara (susceptible), previously shown to have contrasting root growth phenotypes in response to the early phase of salinity stress. GC-MS was used to determine spatial changes in primary metabolites in barley roots in response to salt stress, by profiling three different regions of the root: root cap/cell division zone (R1), elongation zone (R2), and maturation zone (R3). We identified 76 known metabolites, including 29 amino acids and amines, 20 organic acids and fatty acids, and 19 sugars and sugar phosphates. The maintenance of cell division and root elongation in Clipper in response to short-term salt stress was associated with the synthesis and accumulation of amino acids (i.e. proline), sugars (maltose, sucrose, xylose), and organic acids (gluconate, shikimate), indicating a potential role for these metabolic pathways in salt tolerance and the maintenance of root elongation. The processes involved in root growth adaptation and the underlying coordination of metabolic pathways appear to be controlled in a region-specific manner. This study highlights the importance of utilizing spatial profiling and will provide us with a better understanding of abiotic stress response(s) in plants at the tissue and cellular level.
منابع مشابه
Effect of planting time on grain yield and yield components of rainfed barley (Hordeum vulgare L.) genotypes under climatic conditions of Mashhad, Iran
To evaluate grain yield of barley cultivars in different planting times and identifying genotypes suitable fro delayed planting under rainfed conditions, this study was carried out in 2015-2016 and 2016-2017 cropping cycle in Torogh research station in Mashhad, Iran. Two planting times; autumn and dormant seeding and 12 barley genotypes were compared as split plot arrangements in randomized co...
متن کاملTissue Metabolic Responses to Salt Stress in Wild and Cultivated Barley
A thorough understanding of the mechanisms underlying barley salt tolerance and exploitation of elite genetic resource are essential for utilizing wild barley germplasm in developing barley varieties with salt tolerance. In order to reveal the physiological and molecular difference in salt tolerance between Tibetan wild barley (Hordeum spontaneum) and cultivated barley (Hordeum vulgare), profil...
متن کاملMetabolite profiling of barley flag leaves under drought and combined heat and drought stress reveals metabolic QTLs for metabolites associated with antioxidant defense
Barley (Hordeum vulgare L.) is among the most stress-tolerant crops; however, not much is known about the genetic and environmental control of metabolic adaptation of barley to abiotic stresses. We have subjected a genetically diverse set of 81 barley accessions, consisting of Mediterranean landrace genotypes and German elite breeding lines, to drought and combined heat and drought stress at an...
متن کاملVariation in salt tolerance of cultivated (hordeum vulgare L.)and wild (H. spontaneum C. koch)barley genotypes from iran
متن کامل
بررسی اثرات تنش آبی بر برخی خصوصیات فنولوژیکی، فیزیولوژیکی و مورفولوژیکی ژنوتیپ های جو (Hordeum vulgare L.) بهاره دیم
Drought is one of the most destructive abiotic stresses worldwide that affects growth, development and final yield of crops. Reactions of thirteen rainfed spring 2-row barley genotypes and cultivars of cold areas of Iran to water stress were evaluated based on phenological, physiological and morphological traits. The experiment was carried out as factorial experiment based on a randomized com...
متن کامل